Experimental protocols: Difference between revisions
Jump to navigation
Jump to search
| (2 intermediate revisions by the same user not shown) | |||
| Line 3: | Line 3: | ||
==Cloning== | ==Cloning== | ||
*[[Molecular Biology Reagents]] | *[[Molecular Biology Reagents]] | ||
*[[LIC Cloning vectors and | *[[LIC_Cloning_-_May_2010|LIC Cloning vectors and protocols]] | ||
*[[LIC PCR reactions details]] | *[[LIC_PCR_reaction|LIC PCR reactions details]] | ||
==Expression Testing== | ==Expression Testing== | ||
| Line 54: | Line 54: | ||
==Assays in cells== | ==Assays in cells== | ||
==Making | ==Making mismatched DNA== | ||
*[[long linear Mismatched dsDNA]] | *[[long linear Mismatched dsDNA]] | ||
*[[circular Mismatched dsDNA]] | *[[circular Mismatched dsDNA]] | ||
Latest revision as of 13:37, 5 May 2010
Chemical list
Cloning
Expression Testing
- Cell lysis with Spheroplasts in Plates - Grenoble original
- Cell lysis with Spheroplasts in Falcon Tubes - NKI modification
- Running Basic Native Gells - Addapted from The Hebrew University of Jerusalem
E.coli expression
- Competent E.coli
- Minimal media
- PreScission Protease expression and purification
- TEV protease expression and purification
Insect cell expression
Mammalian expression
Purification
Crystallization
- Lysine Methylation
- TECAN tutorial
- Crystallisation Screens and XtalHit
- Hydra II
- Mosquito
- Crystal Farm
- AutoInspex II imaging system
- TOPAZ
- RockImager
Biophysical methods
- MALLS
- ES mass spectrometry
- Biacore
- ITC
- Fluorescence anisotropy
- SMART analytical gel filtration
- Thermal Shift Assays
- Fluorimeter
- ATP Bioluminescence (Luciferase) Assay
- SAXS