Experimental protocols: Difference between revisions
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| Line 54: | Line 54: | ||
==Assays in cells== | ==Assays in cells== | ||
==Making | ==Making mismatched DNA== | ||
*[[long linear Mismatched dsDNA]] | *[[long linear Mismatched dsDNA]] | ||
*[[circular Mismatched dsDNA]] | *[[circular Mismatched dsDNA]] | ||
Latest revision as of 13:37, 5 May 2010
Chemical list
Cloning
Expression Testing
- Cell lysis with Spheroplasts in Plates - Grenoble original
- Cell lysis with Spheroplasts in Falcon Tubes - NKI modification
- Running Basic Native Gells - Addapted from The Hebrew University of Jerusalem
E.coli expression
- Competent E.coli
- Minimal media
- PreScission Protease expression and purification
- TEV protease expression and purification
Insect cell expression
Mammalian expression
Purification
Crystallization
- Lysine Methylation
- TECAN tutorial
- Crystallisation Screens and XtalHit
- Hydra II
- Mosquito
- Crystal Farm
- AutoInspex II imaging system
- TOPAZ
- RockImager
Biophysical methods
- MALLS
- ES mass spectrometry
- Biacore
- ITC
- Fluorescence anisotropy
- SMART analytical gel filtration
- Thermal Shift Assays
- Fluorimeter
- ATP Bioluminescence (Luciferase) Assay
- SAXS