Experimental protocols: Difference between revisions
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==Cloning== | ==Cloning== | ||
*[[Molecular Biology Reagents]] | *[[Molecular Biology Reagents]] | ||
*[[LIC Cloning - May 2010]] | *[[LIC Cloning vectors and procedures - May 2010]] | ||
*[[LIC | *[[LIC PCR reactions details]] | ||
==Expression Testing== | ==Expression Testing== | ||
Revision as of 12:50, 4 May 2010
Chemical list
Cloning
Expression Testing
- Cell lysis with Spheroplasts in Plates - Grenoble original
- Cell lysis with Spheroplasts in Falcon Tubes - NKI modification
- Running Basic Native Gells - Addapted from The Hebrew University of Jerusalem
E.coli expression
- Competent E.coli
- Minimal media
- PreScission Protease expression and purification
- TEV protease expression and purification
Insect cell expression
Mammalian expression
Purification
Crystallization
- Lysine Methylation
- TECAN tutorial
- Crystallisation Screens and XtalHit
- Hydra II
- Mosquito
- Crystal Farm
- AutoInspex II imaging system
- TOPAZ
- RockImager
Biophysical methods
- MALLS
- ES mass spectrometry
- Biacore
- ITC
- Fluorescence anisotropy
- SMART analytical gel filtration
- Thermal Shift Assays
- Fluorimeter
- ATP Bioluminescence (Luciferase) Assay
- SAXS