Experimental protocols

Cloning

• Molecular Biology Reagents
• LIC Cloning vectors and protocols
• LIC PCR reactions details

Expression Testing

• Cell lysis with Spheroplasts in Plates - Grenoble original
• Cell lysis with Spheroplasts in Falcon Tubes - NKI modification
• Running Basic Native Gells - Addapted from The Hebrew University of Jerusalem

E.coli expression

• Competent E.coli
• Minimal media
• PreScission Protease expression and purification
• TEV protease expression and purification

Insect cell expression

• Insect cell expression

Purification

• TCA precipitation
• AchBP purification

Crystallization

• Lysine Methylation
• TECAN tutorial
• Crystallisation Screens and XtalHit
• Hydra II
• Mosquito
• Crystal Farm
• AutoInspex II imaging system
• TOPAZ
• RockImager

Biophysical methods

• MALLS
• ES mass spectrometry
• Biacore
• ITC
• Fluorescence anisotropy
• SMART analytical gel filtration
• Thermal Shift Assays
• Fluorimeter
• ATP Bioluminescence (Luciferase) Assay
• SAXS

In vitro assays

• Phage testing
• In vivo Mismatch repair

Making substrates

• long linear Mismatched dsDNA
• circular Mismatched dsDNA