Experimental protocols
Chemical list
Cloning
Expression Testing
- Cell lysis with Spheroplasts in Plates - Grenoble original
- Cell lysis with Spheroplasts in Falcon Tubes - NKI modification
- Running Basic Native Gells - Addapted from The Hebrew University of Jerusalem
E.coli expression
- Competent E.coli
- Minimal media
- PreScission Protease expression and purification
- TEV protease expression and purification
Insect cell expression
Mammalian expression
Purification
Crystallization
- Lysine Methylation
- TECAN tutorial
- Crystallisation Screens and XtalHit
- Hydra II
- Mosquito
- Crystal Farm
- AutoInspex II imaging system
- TOPAZ
- RockImager
Biophysical methods
- MALLS
- ES mass spectrometry
- Biacore
- ITC
- Fluorescence anisotropy
- SMART analytical gel filtration
- Thermal Shift Assays
- Fluorimeter
- ATP Bioluminescence (Luciferase) Assay
- SAXS